纤维分解酶与异丁酸对犊牛小肠消化酶活力和肝生长轴基因表达的影响

doi:10.11843/j.issn.0366-6964.2016.09.017

Abstract

Abstract:

The objective of this study was to evaluate the effects of fibrolytic enzymes，isobutyrate or their mixture on digestive enzyme activity of small intestine and growth axis hormone receptor gene expression of liver in dairy calves.Thirty-six Holstein male calves of 30 days of age with similar weight were randomly divided into 4 groups.Calves in control group were fed basal ration.Calves in treatments were supplemented fibrolytic enzymes (FE，1.83 g per calf per day)，isobutyrate (IB，98.5% of isobutyrate，6 g per calf per day) and their mixtures (IBFE，FE + IB)，respectively.At 45 and 90 days of age,3 calves from each group were randomly selected and slaughtered.Samples of small intestine contents and liver were collected in the morning before feeding on 45 and 90 days of age for determining intestinal digestive enzyme activity，GHR，IGF-IR and IR mRNA expression in liver.The results showed that on 45 days of age，amylase activities of proximal jejunum digesta，distal jejunum digesta and ileum digesta in IBFE group were significantly higher than FE and control groups(P＜0.05).Trypsin activity of duodenum digesta，proximal jejunum digesta，distal jejunum digesta and ileum digesta in IBFE group were significantly higher than other groups(P＜0.05).Lipase activities of proximal jejunum digesta and distal jejunum digesta in IBFE and IB groups were significantly higher than control group(P＜0.05).The mRNA expression of GHR and IGF-IR in liver in IBFE and IB groups were significantly higher than that of FE and control groups(P＜0.05).The mRNA expression of IR in liver in IBFE group was significantly higher than other groups (P＜0.05).On 90 days of age，amylase activities of duodenum digesta and ileum digesta in IBFE group were significantly higher than that of FE and control groups(P＜0.05).Trypsin activities of proximal jejunum digesta，distal jejunum digesta and ileum digesta in IBFE group were significantly higher than other groups(P＜0.05).Lipase activities of duodenum digesta，proximal jejunum digesta，distal jejunum digesta and ileum digesta in IBFE group were significantly higher than that of FE and control groups(P＜0.05).The mRNA expression of GHR in liver in IBFE，IB and FE groups were significantly higher than that of control group(P＜0.05).The mRNA expression of IR in liver in IBFE group was significantly higher than other groups(P＜0.05).The mRNA expression of IGF-ⅠR of liver in IBFE and IB groups were significantly higher than that of FE and control groups(P＜0.05).Intestinal digestive enzyme activity，mRNA expression of GHR，IGF-IR and IR in liver were improved by the supplementation of the mixture of fibrolytic enzymes and isobutyrate in calves.

CLC Number:

S823.5
S816

ZHANG Ying-ying，WANG Cong，LIU Qiang，ZHANG Yan-li，PEI Cai-xia，WANG Yong-xin，GUO Gang，HUO Wen-jie，ZHNAG Shuan-lin. Effects of Fibrolytic Enzymes and Isobutyrate on Digestive Enzyme Activity of Small Intestine and Growth Axis Hormone Receptor Gene Expression of Liver in Dairy Calves[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(9): 1879-1887.

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Effects of Fibrolytic Enzymes and Isobutyrate on Digestive Enzyme Activity of Small Intestine and Growth Axis Hormone Receptor Gene Expression of Liver in Dairy Calves

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